in vitro azadirachtin production by hairy root cultivation

In vitro azadirachtin production by hairy root cultivation ...

Azadirachtin, a well-known biopesticide is a secondary metabolite conventionally extracted from the seeds of Azadirachta indica. The present study involved in vitro azadirachtin production by developing hairy roots of A. indica via Agrobacterium rhizogenes-mediated transformation of A. indica explants.

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In Vitro Azadirachtin Production by Hairy Root Cultivation ...

10%  Nov 15, 2011  Hence, the growth cycle period for the hairy root culture could be reduced from 40 to 25 days in order to achieve maximum azadirachtin production from the in vitro cultivation of A. indica liquid hairy root culture. Scale-up of A. indica Hairy Root Cultivation in Liquid-Phase and Gas-Phase Reactors Stirred Tank Reactor

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Effect of elicitors and precursors on azadirachtin ...

The present study involved strategies for enhancement in in vitro azadirachtin (commercially used biopesticide) production by hairy root cultivation of Azadirachta indica. Improvement in the azadirachtin production via triggering its biosynthetic pathway in plant cells was carried out by the exogenous addition of precursors and elicitors in the ...

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Azadirachtin production by hairy root cultivation of ...

May 13, 2012  In vitro azadirachtin production by hairy root cultivation of Azadirachta indica in nutrient mist bioreactor. Srivastava S, Srivastava AK. Appl Biochem Biotechnol, 166(2):365-378, 15 Nov 2011 Cited by: 8 articles PMID: 22083394

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Production of the biopesticide azadirachtin by hairy root ...

Production of the biopesticide azadirachtin by hairy root cultivation of Azadirachta indica in liquid-phase bioreactors. Srivastava S(1), Srivastava AK. Author information: (1)Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi,

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Azadirachtin production by hairy root cultivation of ...

May 13, 2012  10%  Present investigation involves hairy root cultivation of Azadirachta indica in a modified stirred tank reactor under optimized culture conditions for maximum volumetric productivity of azadirachtin. The selected hairy root line (Az-35) was induced via Agrobacterium rhizogenes LBA 920-mediated transformation of A. indica leaf explants (Coimbatore variety, India).

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Production of the Biopesticide Azadirachtin by Hairy Root ...

Aug 17, 2013  10%  Batch cultivation of Azadirachta indica hairy roots was carried out in different liquid-phase bioreactor configurations (stirred-tank, bubble column, bubble column with polypropylene basket, and polyurethane foam disc as root supports) to investigate possible scale-up of the A. indica hairy root culture for in vitro production of the biopesticide azadirachtin.

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Production of the biopesticide azadirachtin by hairy root ...

Aug 17, 2013  Batch cultivation of Azadirachta indica hairy roots was carried out in different liquid-phase bioreactor configurations (stirred-tank, bubble column, bubble column with polypropylene basket, and polyurethane foam disc as root supports) to investigate possible scale-up of the A. indica hairy root culture for in vitro production of the biopesticide azadirachtin.

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Effect of Elicitors and Precursors on Azadirachtin ...

Dec 21, 2013  10%  The present study involved strategies for enhancement in in vitro azadirachtin (commercially used biopesticide) production by hairy root cultivation of Azadirachta indica. Improvement in the azadirachtin production via triggering its biosynthetic pathway in plant cells was carried out by the exogenous addition of precursors and elicitors in the growth medium. Among

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List of Publications – Department of Biotechnology

In vitro cultivation of azadirachtin production by hairy root cultivation of Azadirachta indica in nutrient mist bioreactor. Applied Biochemistry and Biotechnology, 166, 365-378, 2012. Applied Biochemistry and Biotechnology, 166, 365-378, 2012.

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Production of the biopesticide azadirachtin by hairy root ...

Aug 17, 2013  Batch cultivation of Azadirachta indica hairy roots was carried out in different liquid-phase bioreactor configurations (stirred-tank, bubble column, bubble column with polypropylene basket, and polyurethane foam disc as root supports) to investigate possible scale-up of the A. indica hairy root culture for in vitro production of the biopesticide azadirachtin.

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Azadirachtin production by hairy root cultivation of ...

May 13, 2012  This included cultivation azadirachtin production was highest on 15th day of the growth cycle (2.96 mg/(l day)) as a result combined addition of a fast-growing, high-yielding hairy root line of A. indica under optimum cultivation conditions in the selected bio- of the elicitor and precursor on 15th day could possibly enhance the azadirachtin ...

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In vitro production of azadirachtin from cell suspension ...

Mar 01, 2008  In vitro azadirachtin production by hairy root cultivation of Azadirachta indica in nutrient mist bioreactor. Srivastava S, Srivastava AK. Appl Biochem Biotechnol, 166(2):365-378, 15 Nov 2011 Cited by: 6 articles PMID: 22083394

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Effect of Elicitors and Precursors on Azadirachtin ...

Dec 21, 2013  The present study involved strategies for enhancement in in vitro azadirachtin (commercially used biopesticide) production by hairy root cultivation of Azadirachta indica. Improvement in the azadirachtin production via triggering its biosynthetic pathway in plant cells was carried out by the exogenous addition of precursors and elicitors in the growth medium. Among the

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Enhanced production of azadirachtin by hairy root cultures ...

Oct 21, 2006  In vitro azadirachtin production by hairy root cultivation of Azadirachta indica in nutrient mist bioreactor. Srivastava S, Srivastava AK. Appl Biochem Biotechnol, 166(2):365-378, 15 Nov 2011 Cited by: 8 articles PMID: 22083394

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Statistical medium optimization for enhanced azadirachtin ...

production of 13.3 gl-1 dry weight. To enhance the production of azadirachtin, a Plackett-Burman experimental design protocol was used to identify key medium nutrients and concentrations to support high root biomass production and azadirachtin accumulation in hairy roots. The optimal nutrients and concentrations were as follows: 40 gF1

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Strategies to Overcome Oxygen Transfer Limitations During ...

Jan 13, 2012  Hence, the growth cycle period for the hairy root culture could be reduced from 40 to 25 days in order to achieve maximum azadirachtin production from the in vitro cultivation of A. indica liquid hairy root culture. Strategies to Overcome the Mass Transfer Limitations in the Hairy Root Cultivation Effect of Oxygen (O 2)

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Azadirachtin technical mixture, analytical standard ...

In vitro azadirachtin production by hairy root cultivation of Azadirachta indica in nutrient mist bioreactor. Smita Srivastava et al. Applied biochemistry and biotechnology, 166(2), 365-378 (2011-11-16)

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List of Publications – Department of Biotechnology

In vitro cultivation of azadirachtin production by hairy root cultivation of Azadirachta indica in nutrient mist bioreactor. Applied Biochemistry and Biotechnology, 166, 365-378, 2012. Applied Biochemistry and Biotechnology, 166, 365-378, 2012.

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Effect of elicitors and precursors on azadirachtin ...

Dec 21, 2013  In vitro azadirachtin production by hairy root cultivation of Azadirachta indica in nutrient mist bioreactor. Srivastava S, Srivastava AK. Appl Biochem Biotechnol, 166(2):365-378, 15 Nov 2011 Cited by: 8 articles PMID: 22083394

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Smita SRIVASTAVA Professor (Associate) PhD Indian ...

The present study involved strategies for enhancement in in vitro azadirachtin (commercially used biopesticide) production by hairy root cultivation of Azadirachta indica.

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Hairy Root Culture: Bioreactor Design and Process ...

Apr 19, 2013  Srivastava S, Srivastava AK (2012b) In vitro Azadirachtin production by hairy root cultivation of Azadirachta indica in nutrient mist bioreactor. Appl

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*PUBLICATIONS*

Smita Srivastava, "In vitro azadirachtin (biopesticide) production by hairy root cultivation of Azadirachta indica in a modified bubble column reactor", Invited paper presentation under ISCA Young Scientists Award Programme for 2011-12 in Engineering Sciences, October 14, 2011, KIIT University, Bhubaneswar, India.

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(PDF) Understanding the Role of Cytokinins in Tissue ...

Tissue proliferation (TP) is characterized primarily by the formation of galls or tumors at the crown of container-grown rhododendrons propagated in vitro. However, TP of Rhododendron `Montego#39; is observed initially in in vitro shoot cultures and

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Statistical medium optimization for enhanced azadirachtin ...

production of 13.3 gl-1 dry weight. To enhance the production of azadirachtin, a Plackett-Burman experimental design protocol was used to identify key medium nutrients and concentrations to support high root biomass production and azadirachtin accumulation in hairy roots. The optimal nutrients and concentrations were as follows: 40 gF1

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Production of biopesticide Azadirachtin by Hairy Root of A ...

View Production of biopesticide Azadirachtin by Hairy Root of Adica in Liquid phase bioreactors.pdf from BIOTECH 15BT201 at SRM University. Appl Biochem Biotechnol (2013) 171:1351–1361 DOI

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*PUBLICATIONS*

Smita Srivastava, "In vitro azadirachtin (biopesticide) production by hairy root cultivation of Azadirachta indica in a modified bubble column reactor", Invited paper presentation under ISCA Young Scientists Award Programme for 2011-12 in Engineering Sciences, October 14, 2011, KIIT University, Bhubaneswar, India.

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Mass production of Ajmalicine by bioreactor cultivation of ...

Mar 15, 2017  In vitro azadirachtin production by hairy root cultivation of azadirachta indica in nutrient mist bioreactor Appl. Biochem. Biotechnol. , 166 ( 2012 ) , pp. 365 - 378

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Hairy Root Culture: Bioreactor Design and Process ...

Apr 19, 2013  Srivastava S, Srivastava AK (2012b) In vitro Azadirachtin production by hairy root cultivation of Azadirachta indica in nutrient mist bioreactor. Appl

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First Report of Agrobacterium rhizogenes-induced Hairy ...

The hairy root culture (HRC)-based in vitro productions were reported for bioactive molecules such as shikonins, azadirachtin, camptothecins, harpagide, ginsenosides, paclitaxel, hyoscyamine and scopolamine [1 1 Satdive RK, Fulzele DP, Eapen S. Enhanced production of azadirachtin by hairy root cultures of Azadirachta indica A. Juss by ...

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Plant Cell-Batch Cultivation (Theory) : Bioreactor ...

Hence, in vitro cultivation of plant cells in bioreactor is considered to be an attractive alternative and viable approach for the production of these compounds. Bio-pesticide production from A. indica . Azadirachtin is presently being isolated by solvent extraction from the seed kernels of A. indica.

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» Smita Srivastava Department of BiotechnologyBhupat and ...

S. Srivastavaand A. K. Srivastava (2012) In vitro cultivation of azadirachtin production by hairy root cultivation of Azadirachta indica in nutrient mist bioreactor Applied Biochemistry and Biotechnology. 166:365-378. SrivastavaSmita (2011) In vitro production of therapeutic cyclotides via plant cell bioprocessing. Editorial in Res. J. Biotech ...

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azadirachtin, 11141-17-6

PubMed:In vitro azadirachtin production by hairy root cultivation of Azadirachta indica in nutrient mist bioreactor. PubMed:In vitro and in vivo antiparasitic activity of Azadirachtin against Argulus spp. in Carassius auratus (Linn. 1758).

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Smita SRIVASTAVA Professor (Associate) PhD Indian ...

The present study involved strategies for enhancement in in vitro azadirachtin (commercially used biopesticide) production by hairy root cultivation of Azadirachta indica.

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strategies to overcome oxygen transfer limitation during ...

View strategies to overcome oxygen transfer limitation during Hairy Root cultivations of Azadiracta indic from BIOLOGY 10 at SRM Kattankulathur Dental College Hospital , Kancheepuram. Appl Biochem

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Hairy Root Culture - an overview ScienceDirect Topics

A. tumefaciens and A. rhizogenes infected root furnish a promising platform for the genetic manipulation of plant cells. As discussed A. rhizogenes-mediated transformation of plants can be used in a similar manner to that used during A. tumefaciens transformation. Hairy root syndrome and production of transgenic hairy root cultures by genetic manipulation can be achieved by A. rhizogenes ...

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Production of Limonoids with Insect Antifeedant Activity ...

Neem tree (Azadirachta indica) cell suspension culture is an alternative for the production of limonoids for insect control that overcomes limitations related to the supply of neem seeds. To establish conditions for cell growth and azadiracthin-related limonoid production, the effect of different sucrose concentrations, nitrate and phosphate in Murashige and Skoog (MS) medium, and the addition ...

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Effect of α-glycosidase inhibitors from endophytic fungus ...

Aug 06, 2019  In the present study the production of α-glycosidase inhibitors was used as a strategy to screen endophytic fungi with insecticidal and antifungal potential. Endophytic fungi were isolated from ...

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